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Suppressyn (131-160) / HERV-Fb1 (131-160) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-059-52 50 µl $450.00

Image

 

Protocol for Immunohistochemistry: 

 Tissue Sample Human Placenta tissue
 Fixative 4% Paraformaldehyde 8 hours
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Intact
 Blocking 10% Normal Goat Serum
 Primary Antibody Rabbit Anti-Suppressyn (131-160) (Human)(Cat No.:H-035-56)
 Optimal Dilution 1:100 (1 hour at 37°C and overnight 4°C)
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (Vector Laboratory) (37°C 60 min)
 Amplification  ABC (Vector) (30 min)
 Detection system HRP
 Substrate DAB (Vector), 2 min
 Counterstained Hematoxylin, 30 Sec
Catalog #H-059-52
Standard Size50 µl
SpeciesHuman
HostRabbit
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