C19orf10 / MYDGF Precursor (32-98)-amide (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample	Human Placenta
Fixative	4% Paraformaldehyde 8 hours
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	Intact
Blocking	10% Normal Goat Serum (37 °C, 60 min)
Primary Antibody	MYDGF precursor (32-98)-amide (Human)(Cat No.:H-056-60)
Optimal Dilution	1: 100 (1hour at 37 °C and overnight 4 °C)
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (Vector Laboratory) (37 °C, 60 min)
Amplification	ABC (Vector Laboratory) (30 min)
Detection system	HRP
Substrate	DAB (Vector Laboratory), 2 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-056-60

Standard Size

50 µl

Species

Human

Host

Rabbit

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