Synaptotagmin IV (73-97) (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample	Human brain tissues
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Synaptotagmin IV (73-97) (Human) Antibody (Catalog No.: H-008-64)
Optimal Dilution	1:500, 1 hour at RT
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-008-64

Family

Synaptotagmin IV

Standard Size

100 µl

Sequence

Phe - Gly - Ala - Asp - Asp - Lys - Asn - Glu - Val - Lys - Asn - Lys - Pro - Ala - Val - Pro - Lys - Asn - Ser - Leu - His - Leu - Asp - Leu - Glu

Species

Human

Host

Rabbit

Immunogen

Synaptotagmin IV (73-97) (Human)

Reconstitution

For best and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Recommended Dilution Factor

Immunohistochemistry - 1:500

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