Orexin A (16-33) Amide (Human, Rat, Mouse, Porcine, Ovine, Monkey)

Image

Protocol for Immunohistochemistry:
Tissue Sample	Human Pancreas, Mouse/Rat Stomach tissues
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit anti-Orexin A (16-33) (Human, Rat) antibody (Cat. No.: H-003-36)
Optimal Dilution	1:500
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-003-36

Family

Orexin A

Standard Size

50 µl

Sequence

Leu - Tyr - Glu - Leu - Leu - His - Gly - Ala - Gly - Asn - His - Ala - Ala - Gly - Ile - Leu - Thr - Leu - NH2

Species

Human, Rat, Mouse, Porcine, Bovine, Ovine

Specific Specie

Monkey

Host

rabbit

Immunogen

Orexin A (16-33) Amide (Human, Rat, Mouse, Porcine, Ovine)

Reconstitution

For consistent reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Alphabet_O, H_