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H-035-30
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INSL 6 / RLF / RIF-1 (Human) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-035-30
50 µl
$450.00
Relaxin & Relaxin-Related Peptides
Insulin-like 6 Peptide (INSL 6)
Relaxin Evolution
GSS: Relaxin Like Peptide
Antibody Production & Evaluation
Image
Protocol for Immunohistochemistry:
Tissue Sample
Human testis
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No primary antibody
Pretreatment
Target retrieval 25 min (Steam)
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-INSL 6 (Human) Antiserum (Catalog No.:H-035-30)
Optimal Dilution
1:2000 (1hour at RT)
Secondary Antibody
Goat anti-Rabbit IgG, Biotinylated (Vector) (1:400), 30 min
Amplification
ABC (Vector) (1:400, 30 min)
Detection system
HRP
Substrate
DAB (Sigma)
Counterstained
Hematoxylin, 30 sec
Catalog #
H-035-30
Standard Size
50 µl
Species
Human
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Alphabet_I_
Please click
here
or click the reference tab on the top to see more articles that use this product.
Links to publications that use this antibody:
Brailoiu et al. Insulin-like 6 immunoreactivity in the mouse brain and testis.
Brain Res. 2005 Apr 8;1040(1-2):187-90.
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