Western Blot Analysis
Protocol for Immunohistochemistry:
Tissue Sample
Rat & Mouse Brain
Fixative
10% formalin
Embedding
Paraffin
Negative Control
No primary antibody
Pretreatment
Intact
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-NPFF1R (185-203) (H) Serum (Catalog No.:H-001-58)
Optimal Dilution
1:200 (1 hour at RT)
Secondary Antibody
Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection System
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec
Links to the publications that use this antibody:
Li X, Su J, Fang R, et al. The effects of RFRP-3, the mammalian ortholog of GnIH, on the female pig reproductive axis in vitro. Mol Cell Endocrinol. 2013
Li X, Su J, Lei Z, et al. Gonadotropin-inhibitory hormone (GnIH) and its receptor in the female pig: cDNA cloning, expression in tissues and expression pattern in the reproductive axis during the estrous cycle. Peptides. 2012;36(2):176-85.