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H-072-15
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CRAMP (140-173) (Mouse) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-072-15
100 µl
$295.00
CRAMP (Mouse) Antibody
Antibody Production & Evaluation
Image
Protocol for Immunohistochemistry:
Tissue Sample
Human lung cancer & Rat/Mouse lung tissue
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No primary antibody
Pretreatment
Target retrieval 25 min (Steam)
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-CRAMP (Mouse) Antibody (Catalog No.:H-072-15)
Optimal Dilution
1:500
Secondary Antibody
Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec
Catalog #
H-072-15
Family
CRAMP
Standard Size
100 µl
Sequence
Gly - Leu - Leu - Arg - Lys - Gly - Gly - Glu - Lys - Ile - Gly - Glu - Lys - Leu - Lys - Lys - Ile - Gly - Gln - Lys - Ile - Lys - Asn - Phe - Phe - Gln - Lys - Leu - Val - Pro - Gln - Pro - Glu - Gln
Species
Mouse
Host
Rabbit
Immunogen
CRAMP (Mouse)
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
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%CRAMP%
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Thursday 15 August, 2024
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