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H-059-02
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PYY (3-36) (Human) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-059-02
100 µl
$450.00
PYY
PYY Immunoassay Kits
PYY Sequence Comparison
Normal Plasma Peptide/Protein Levels
PYY Antibodies
Antibody Production & Evaluation
Image
Protocol for Immunohistochemistry:
Tissue Sample
Mouse and rat intestine tissue
Fixative
10% formalin
Embedding
paraffin
Negative Control
Pre-immuno serum
Pretreatment
Intact
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-PYY (3-36) (Human) Serum (Catalog No.:H-059-02)
Optimal Dilution
1:200~500 (1hour at RT)
Secondary Antibody
Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min
Amplification
ABC (Vector) (1:400, 30 min)
Detection System
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec
Western Blot Analysis of PYY in rat intestine tissue
Tissue Sample
Rat intestine tissue homogenate
Electrophoresis
15% Tricine-PAGE gel
Negative control
No primary antibody
Blocking
1% BSA in TBST or 5% dry milk in TBST
Primary Antibody
Rabbit Anti-PYY (3-36) (H) purified IgG (Catalog No.:G-059-02)
Optimal Dilution
1:200 ( 5 ug /ml) (4 hour at RT, or overnight at 4
0
C)
Secondary Antibody
Donkey anti-Rabbit IgG whole (H+L), HRP conjugate(1:5000, 30 min)
Detection system
HRP
Substrate
SuperSignal Chemiluminescent Substrate
Catalog #
H-059-02
Standard Size
100 µl
Sequence
Ile - Lys - Pro - Glu - Ala - Pro - Gly - Glu - Asp - Ala - Ser - Pro - Glu - Glu - Leu - Asn - Arg - Tyr - Tyr - Ala - Ser - Leu - Arg - His - Tyr - Leu - Asn - Leu - Val - Thr - Arg - Gln - Arg - Tyr - NH2
Species
Human
Host
Rabbit
Reconstitution
For best and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content
This vial contains 100µl of Rabbit Anti- Peptide YY (3-36) (Human) Serum in the lyophilized form.
Recommended Dilution Factor
ABC: 1:200~500
Alphabet_P_
Please click
here
or click the reference tab on the top to see more articles that use this product.
Links to publications that use this antibody:
Glavas et al. Characterization of brainstem peptide YY (PYY) neurons.
J Comp Neurol. 2008 Jan 10;506(2):194-210.
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