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H-017-03
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Amylin (Human) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-017-03
50 µl
$175.00
Amylin
Normal Plasma Peptide/Protein Levels
Antibody Production & Evaluation
Image
Protocol for Immunohistochemistry
Tissue Sample
Human Pancreas tissue
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No primary antibody
Pretreatment
Target retrieval 10 min (Steam)*
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-Amylin (Human) Purified IgG (Catalog No:H-017-03)
Optimal Dilution
1:200 (1hour at RT)
Secondary Antibody
Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec
* Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Catalog #
H-017-03
Standard Size
50 µl
Sequence
Lys - Cys - Asn - Thr - Ala - Thr - Cys - Ala - Thr - Gln - Arg - Leu - Ala - Asn - Phe - Leu - Val - His - Ser - Ser - Asn - Asn - Phe - Gly - Ala - Ile - Leu - Ser - Ser - Thr - Asn - Val - Gly - Ser - Asn - Thr - Tyr - NH2
Species
Human
Host
Rabbit
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content
This vial contains 50µl of Rabbit Anti-Amylin (Human) Antibody Serum in the lyophilized form.
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