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H-015-09
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CGRP (Rat, Mouse) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-015-09
50 µl
$175.00
Calcitonin Gene Related Peptide (CGRP)
Normal Plasma Peptide/Protein Levels
Antibody Production & Evaluation
Image
Protocol for
Immunohistochemistry
Tissue Sample
Rat heart
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No primary antibody
Pretreatment
Intact
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-CGRP (Rat) Antiserum (Catalog No.:H-015-09)
Optimal Dilution
1:200 (1hour at RT)
Secondary Antibody
Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec
Catalog #
H-015-09
Standard Size
50 µl
Sequence
Ser - Cys - Asn - Thr - Ala - Thr - Cys - Val - Thr - His - Arg - Leu - Ala - Gly - Leu - Leu - Ser - Arg - Ser - Gly - Gly - Val - Val - Lys - Asp - Asn - Phe - Val - Pro - Thr - Asn - Val - Gly - Ser - Glu - Ala - Phe - NH2
Disulfide Bridge:
Cys2 - Cys7
Species
Rat, Mouse
Host
Rabbit
Reconstitution
For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content
This vial contains 50µl of Rabbit Anti-CGRP (Rat) Serum in the lyophilized form.
Recommended Dilution Factor
Immunohistochemistry - 1:1000.
(BMC Physiol. 2007 Aug 28;7:9.)
Cross Reactivity
Peptide
% Cross-reactivity
CGRP (Rat)
100
CGRP II (Rat)
78.6
CGRP (Human)
35.5
CGRP II (Human)
16.8
Amylin (Rat)
<0.01
Amylin (Human)
<0.001
Calcitonin (Rat)
0
Somatostatin-14
0
Alphabet_C_
Please click
here
or click the reference tab on the top to see more articles that use this product.
Links to publications that use this antibody:
Zvara et al. Exogenous overexpression of nerve growth factor in the urinary bladder produces bladder overactivity and altered micturition circuitry in the lumbosacral spinal cord
BMC Physiol. 2007 Aug 28;7:9.
Zhou et al. Plastic changes of calcitonin gene-related peptide in morphine tolerance: behavioral and immunohistochemical study in rats.
J Neurosci Res. 2003 Nov 15;74(4):622-9.
Xu et al. Antinociceptive effect of calcitonin gene-related peptide in the central nucleus of amygdala: activating opioid receptors through amygdala-periaqueductal gray pathway.
Neuroscience. 2003;118(4):1015-22.
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