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H-071-22
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Urotensin II, Prepro (71-84) (Human) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-071-22
100 µl
$450.00
Urotensin II
Antibody Production & Evaluation
Image
Protocol for Immunohistochemistry
Tissue Sample
Mouse & rat brain tissue
Fixative
10% Formalin
Embedding
Paraffin
Negative control
Pre-immuno serum
Pretreatment
Intact
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-urotensin II, Prepro (71-84) (Human) Serum (Catalog No.:H-071-22)
Optimal Dilution
1:500 (over night at 4°C)
Secondary Antibody
Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec
Western Blot Analysis of Prepro Urotensin II in rat brain tissue
Tissue Sample
Rat brain tissue homogenate
Electrophoresis
15% Tricine-PAGE gel
Negative control
No primary antibody
Blocking
1% BSA in TBST or 5% dry milk in TBST
Primary Antibody
Rabbit Anti-Urotensin, Prepro (71-84)(Human) Antiserum (Catalog No.:H-071-22)
Optimal Dilution
1:500 (4 hour at RT, or overnight at 4
0
C)
Secondary Antibody
Donkey anti-Rabbit IgG whole (H+L), HRP conjugate(1:5000, 30 min)
Detection system
HRP
Substrate
SuperSignal Chemiluminescent Substrate
Catalog #
H-071-22
Standard Size
100 µl
Sequence
Ala - Asp - Ser - Ser - Thr - Asn - Ile - Phe - Asn - Pro - Arg - Gly - Asn - Leu
Species
Human
Host
Rabbit
Reconstitution
For consistent and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum immediately before use.
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content
This vial contains 100µl of rabbit anti-Urotensin II, Prepro (71-84) (Human) serum in the lyophilized form.
Recommended Dilution Factor
Direct ELISA: 1:3200
Immonohistochemistry (ABC) 1:500
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