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H-028-54A
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Adiponectin Precursor [Cys0] (223-244) (Rat, Mouse) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-028-54A
100 µl
$275.00
Adiponectin, Adiponectin Receptors & Adiponectin EIA Kits
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Antibody Production & Evaluation
Image
Protocol for Western Blot:
Sample
Rat serum, plasma, visceral fat tissue
Electrophoresis
10% SDS-PAGE gel, reducing condition
Denatured sample
100'C for 5 min
Positive control
Adiponectin (223-244), Cys0, (Rat) peptide
Blocking
1% BSA in TBST
Primary Antibody
Rabbit Anti-Adiponectin (222-244) (Rat) Antiserum (Catalog No.:H-028-54, Lot No.: PB105-2)
Optimal Dilution
1:500 (overnight at cold room)
Secondary Antibody
Rabbit anti-Mouse IgG, HRP conjugated (1: 12500)
Substrate
ECL
Positive Band
31 ~32 KDa
Protocol for Immunohistochemistry:
Tissue Sample
Rat visceral white fat tissue
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No primary antibody
Pretreatment
Intact
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-Adiponectin (223-244) (Rat) Antiserum (Catalog No.:H-028-54, Lot No.: PB105-2)
Optimal Dilution
1:500~1000 (overnight hour at 4
0
C)
Secondary Antibody
Goat anti-Rabbit IgG, HRP conjugated
Amplification
ABC (Vector)
Detection system
HRP
Substrate
DAB (Sigma)
Counterstained
Hematoxylin
Catalog #
H-028-54A
Standard Size
100 µl
Species
Rat
Host
Rabbit
Reconstitution
Reconstitute with 100 ul or 200 ul distill water or PBS
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Recommended Dilution Factor
1: 3200 ( indirect ELISA )
1: 500 ~ 1000 (Western blot analysis)
1: 500 ~ 1000 (Immunohistochemistry)
Cross Reactivity
This antibody is crossreactivity with rat adiponectin (223-244). It also tested in rat white fat tissue in western blot analysis and immunohistmochemistry.
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