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H-005-99
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Neuropeptide S, Prepro (23-67) (Mouse) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-005-99
100 µl
$350.00
Neuropeptide S & N-terminal NPS
Antibody Production & Evaluation
Image
Protocol for Immunohistochemistry
Tissue Sample
Rat and Mouse Brain Tissue
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No primary antibody
Pretreatment
Target Retrieval 25 min (Steam)
Blocking
2% Normal Goat Serum, 3%H
2
O
2
Primary Antibody
Rabbit anti-Neuropeptide S, Prepro (23-67) Serum (Catalog No.: H-005-99)
Optimal Dilution
1:500 (1 hour at RT )
Secondary Antibody
Goat Anti-Rabbit IgG, Biotinylated (1:400)
Amplification
ABC (Vector)
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin
Catalog #
H-005-99
Family
Neuropeptide S
Standard Size
100 µl
Sequence
Cys - Tyr - Pro - Val - Leu - Ser - Ser - Lys - Val - Pro - Gly - Lys - Pro - Asp - Tyr - Phe - Leu - Ile - Leu - Leu - Ser - Ser - Cys - Pro - Ala - Arg - Leu - Glu - Gly - Ser - Asp - Arg - Leu - Ala - Phe - Leu - Lys - Pro - Ile - Leu - Glu - Lys - Thr - Ser - Met Disulfide bridge Cys23 - Cys45
Species
Mouse
Host
Rabbit
Immunogen
Neuropeptide S, Prepro (23-67) (Mouse)
Reconstitution
100 ul
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Recommended Dilution Factor
Immunohistochemistry (Rat Brain) - 1:200
Immunohistochemistry (Mouse Brain) - 1:500
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