Adiponectin, Adiponectin Receptors & Adiponectin EIA Kits

Adiponectin Receptor-1 &-2 (AdipoR1 & AdipoR2) two recently identified receptors

Normal Plasma Peptide/Protein Levels

Predict Alzheimer's Disease up to 10 years in advance

Catalog #	ADI-01B
Family	adiponectin
Standard Size	25 µg
Sequence	AA Sequence: GEGAY VYRSAFSVGL ETYVTIPNMP IRFTKIFYNQ QNHYDGSTGK FHCNIPGLYY FAYHITVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTN Source: E.Coli
Species	Human
M.W	16.6 KD
Solubility	The lyophilized adiponectin (human) should be reconstituted in water to a concentration of <0.3mg/ml. Allow to set at least 30 minutes at 4-8?C, mix well. If desired, you can centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. This solution can be diluted into other buffers or stored at 4?C for up to 1 week and at -20?C for future use.
Storage Condition	The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20˚C. Reconstituted Adiponectin should be stored in working aliquots at -20˚C. AVOID FREQUENT FREEZE THAW CYCLES.
Content	This vial contains 25 µg of peptide.

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Lot No.:          426843

Product:         Adiponectin Recombinant, Globular Form (Human)

                       

Molecular Weight:    19 kDa

Peptide Contents:

                        This vial contains 100 mg of  protein.

Supplied:        The sterile filtered solution was lyophilized from 20mM Tris, 20 mM NaCl, pH 8.0

Source:           E.Coli

Tag:                6 x His Tag on N-Terminal             

Purity:            >95% by SDS-Page analysis

 

Solubility and Reconstitution:

The lyophilized adiponectin (human) should be reconstituted in water to a concentration of 100 µg/ml. Allow to set at least 30 minutes at 4-8˚C, mix well.

Storage:          The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20˚C to -70˚C. Reconstituted Adiponectin can be stored at 4˚C for one week or  in working aliquots at -20˚C to

                        -70˚C for long term storage.

                        AVOID FREQUENT FREEZE THAW CYCLE.

 Application:

                        ELISA, Western Blot Analysis, Cell Biology.

 

  Adiponectin is very important adipokine involved in the control of fat metabolism and

   Insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory

   activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal

   muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes α-TNF

   by negatively regulating its expression in various tissues such as liver and

   macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B

  signaling through a c-AMP-dependent pathway. May play a role in cell growth,

  angiogenesis and tissue remodeling by binding and sequestering various factors with

  distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.

Precautions: For In vitro research Use Only. Not for use in or on humans of animals or for diagnostics.