Visfatin / PBEF, recombinant (Human)

Catalog #	003-81
Standard Size	10 µg
Sequence	A DNA sequence encoding the human Visfatin (Met 1 - His 491) was fused with a 6 x histidine tag at the C - Terminus. The fused protein was expressed in E. Coli. M.W. ~55 KDa.
Species	Human
M.W	Insulin-mimetic effects on differentiating 3T3-L1 cells
Mass Spec Analysis	Lyophilized from 0.2 µm filtered solution in 10mM Tris, pH 9.2.
Storage Condition	The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20˚C. Reconstituted human Visfatin should be stored in working aliquots at -20˚C. AVOID FREQUENT FREEZE THAW CYCLES. If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10μg/ml. Precautions: For research use Only. Not for use in human.
Content	This vial contains 10 µg of Visfatin (Human), recombinant.
Counter_Ion	0. 1 EU/μg (LAL test)
HPLC Analysis	Purity ≥95% by SDS-Page analysis

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Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that used this protein:

Wanecq et al. Lack of direct insulin-like action of visfatin/Nampt/PBEF1 in human adipocytes.
J Physiol Biochem. 2009 Dec;65(4):351-9.

Dahl et al. Increased expression of visfatin in macrophages of human unstable carotid and coronary atherosclerosis: possible role in inflammation and plaque destabilization.
Circulation. 2007 Feb 27;115(8):972-80.