Endothelin and Endothelin-like Peptides

Preproendothelin-1: Placenta, Lung, Umbillical Vein (Human)

Endothelin-1 Precursor (ET-1) Sequence

Preproendothelin-1 (Porcine)

Normal Plasma Peptide/Protein Levels

Catalog #	023-01
Standard Size	100 µg
Sequence	Cys - Ser - Cys - Ser - Ser - Leu - Met - Asp - Lys - Glu - Cys - Val - Tyr - Phe - Cys - His - Leu - Asp - Ile - Ile - Trp Disulfide Bridge: Cys1 - Cys15 and Cys3 - Cys11
Species	Human, Rat, Mouse, Bovine, Canine, Porcine
Specific Specie	Rabbit, Monkey
M.W	2491.94
Mass Spec Analysis	Exhibits correct M.W.
Solubility	Before reconstitution, please allow 25 to 45 minutes for the lyophilized peptide to return to room temperature. Briefly vortex the Eppendorf tube to concentrate all material at the bottom of the tube. Gently open the cap and reconstitute the peptide to the desired concentration by adding a sterile, aqueous solution to the tube. Invert the tube 5 times by hand to ensure a homogeneous solution.
Storage Condition	Store peptide in DRY form at 0-5°C.
Appearance	White powder
Content	The contents of this vial have been accurately determined to contain 100µg of NET peptide.
HPLC Analysis	Purity ≥95%
Additional articles	For best results and reproducibility, rehydrate peptide just before use. Do not attempt to weigh out smaller portions. Do not refreeze any unused portions.

Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this peptide:

Schellenberg et al. Analytical validation of commercial immunoassays for the measurement of cardiovascular peptides in the dog.
Vet J. 2008 Oct;178(1):85-90.

Thaete et al. Expression of Endothelin 1 and Its Receptors in the Hypoxic Pregnant Rat
Biol Reprod. 2007 Sep;77(3):526-32.

Piuhola et al. Endothelin-1 and angiotensin II contribute to BNP but not c-fos gene expression response to elevated load in isolated mice hearts
Biochim Biophys Acta. 2007 Mar;1772(3):338-44.

Ito et al. Intestinal Microcirculatory Dysfunction During the Development of Experimental Necrotizing Enterocolitis
Pediatr Res. 2007 Feb;61(2):180-4

Sattler et al. Dietary reversal of experimental hypercholesterolemia improves endothelial dysfunction of epicardial arteries but not of small coronary vessels in pigs.
Atherosclerosis. 2006 Oct;188(2):301-8.

Disa et al. Identification and characterization of binding sites for human urotensin-II in Sprague-Dawley rat renal medulla using quantitative receptor autoradiography.
Peptides. 2006 Jun;27(6):1532-7.

Piuhola et al. Dual role of endothelin-1 via ETA and ETB receptors in regulation of cardiac contractile function in mice
Am J Physiol Heart Circ Physiol. 2003 Jul;285(1):H112-8.

Tsuruda et al. Brain natriuretic Peptide is produced in cardiac fibroblasts and induces matrix metalloproteinases.
Circ Res. 2002 Dec 13;91(12):1127-34.

Best et al. Dendroaspis natriuretic peptide relaxes isolated human arteries and veins.
Cardiovasc Res. 2002 Aug 1;55(2):375-84.

Tsuruda et al. Cardiotrophin-1 stimulation of cardiac fibroblast growth: roles for glycoprotein 130/leukemia inhibitory factor receptor and the endothelin type A receptor.
Circ Res. 2002 Feb 8;90(2):128-34.

Wilson et. al - Simvastatin Preserves Coronary Endothelial Function in Hypercholesterolemia in the Absence of Lipid Lowering
Arterioscler Thromb Vasc Biol. 2001 Jan;21(1):122-8.

Howard et al. Identification of receptors for neuromedin U and its role in feeding.
Nature. 2000 Jul 6;406(6791):70-4.

Liang et al. Endothelin-dependent and -independent components of strain-activated brain natriuretic peptide gene transcription require extracellular signal regulated kinase and p38 mitogen-activated protein kinase.
Hypertension. 2000 Jan;35(1 Pt 2):188-92.

Hasdai et al. Enhanced Endothelin-B-Receptor–Mediated Vasoconstriction of Small Porcine Coronary Arteries in Diet-Induced Hypercholesterolemia
Arterioscler Thromb Vasc Biol. 1997 Nov;17(11):2737-43.

For best results and reproducibility, rehydrate peptide just before use. Do not attempt to weigh out smaller portions. Do not refreeze any unused portions.