Description:
SAG is a highly potent agonist of SMO (smoothened). This small molecule compound activates the hedgehog pathway by directly binding to SMO. Note: in the Gli-1 reporter gene assay, SAG acts as an activator at concentrations from approximately 1 nM to 1 uM, but as an inhibitor atconcentrations high than 1 uM.
Ref: Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 14071
Biological Activity:
In a cell-based assay measuring the activation of the Gli-1 reporter gene, this compound gives EC50 of 5-15 nM.
