MGF (mechano-growth factor)

IGF-I (Insulin-Like Growth Factor I)

Vesiculin

Catalog #	033-23
Standard Size	20 µg
Sequence	Ala - Tyr - Arg - Pro - Ser - Glu - Thr - Leu - Cys - Gly - Gly - Glu - Leu - Val - Asp - Thr - Leu - Gln - Phe - Val - Cys - Gly - Asp - Arg - Gly - Phe - Tyr - Phe - Ser - Arg - Pro - Ala - Ser - Arg - Val - Ser - Arg - Arg - Ser - Arg - Gly - Iso - Val - Glu - Glu - Cys - Cys - Phe - Arg - Ser - Cys - Asp - Leu - Ala - Leu - Leu - Glu - Thr - Tyr - Cys - Ala - Thr - Pro - Ala - Lys - Ser - Glu
Species	Human
Content	This vial contains 20µg of peptide.
HPLC Analysis	Purity >98% by SDS-Page gel and HPLC analyses.

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Description:
IGF's are mitogenic polypeptide growth factors. That stimulate the proliferation and survival of various cell types including muscle, bone and cartilage tissue in vitro. IGFs are predominantly produced by liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but, IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant human IGF-I and IGF-II are globular proteins containing 70 (M.W 7.6 kDa) and 67 (M.W. 7.5 kDa) amino acids, respectively, and 3 intra-molecular disulfide bonds.

Source: E.Coli

Endotoxin Level: <1EU/μg

Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1 - 1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at 4˚C for 1 week or at -20˚C for future use.

Stability: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20^oC. Reconstituted IGF-II should be stored in working aliquots at -20^oC.

Biological Activity:  The ED50 was determined by a cell proliferation using FDC-P1 cells and is ≤2.0 ng/ml, corresponding to a specific activity of ≥5x105 units/mg.

Precautions: For In vitro research Use Only. Not for use in or on humans of animals or for diagnostics.