Apelin-13, [pGlu1, Ala13] (Human, Rat, Mouse, Bovine) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample	Human Kidney Tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Apelin-13 [pGlu1, Ala13] (Human, Rat, Mouse, Bovine) Serum (Catalog No.: H-057-30)
Optimal Dilution	1:800 (1 hour at RT)
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma)
Counterstained	Hematoxylin

Catalog #

H-057-30

Family

Apelin

Standard Size

100 µl

Sequence

pGlu - Arg - Pro - Arg - Leu - Ser - His - Lys - Gly - Pro - Met - Pro - Ala

Species

Human, Mouse, Rat, Bovine

Host

Rabbit

Immunogen

Apelin-13 [pGlu1, Ala13] (Human, Rat, Mouse, Bovine)

Reconstitution

For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

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