Wnt-5a (Human)

SFRP-5 and WNT-5a

SFRP-2

Novel Paradigm of Metabolic Regulation

Catalog #	080-03A
Family	WNT
Standard Size	5 µg
Species	Human
M.W	~45 kDa
Solubility	Before reconstitution, we recommend a brief spin to drive down any material dislodged from the bottom of the tube. The lyophilized protein should be reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of this protein, further dilutions should be made in buffer or medium containing carrier proteins, such as albumin or serum.
Storage Condition	The lyophilized protein is stable for at least 6 months if stored at -80 degree C. Reconstituted protein is stable for at least 2 weeks at 4 degree C, but should be stored in aliquots at -80 degree C for longer term. Avoid repeated freeze/thaw.
Appearance	Lyophilized in sterile filtered solution of PBS with 1% CHAPS
HPLC Analysis	Purity is greater than 85% as determined by SDS-PAGE and HPLC analysis

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Synonyms:
Wingless-type MMTV (mouse mammary tumor virus) integration sitefamily member 5a

Description:
WNT-5a belongs to the class of WNT proteins that activate the “noncanonical”pathway. The predicted size of human WNT-5a is a monomericprotein containing 357 amino acid residues. Due to glycosylation, itmigrates at an apparent molecular weight of ~45 kDa on SDS-PAGE undernon-reducing conditions. StemRD’s product is expressed from a humancell line in animal-free medium, and purified with a proprietary processthat is distinct from the published method.

Biological Activity:
The activity was determined by using a TCF reporter gene assay in 293cells transfected with Frizzled-4 and LRP-5. WNT-5a activates (instead ofinhibits) the TCF reporter gene in this assay (Milkels AJ, et al., PLoS Biol,4:e115, 2006).