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080-04A
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WNT-5a, mouse
Catalog#
Standard size
Price
Order
080-04A
5 µg
$245.00
SFRP-2
Novel Paradigm of Metabolic Regulation
Catalog #
080-04A
Standard Size
5 µg
Species
Mouse
M.W
~ 45 kDa
Solubility
Before reconstitution, we recommend a brief spin to drive down any material dislodged from the bottom of the tube. The lyophilized protein should be reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of this protein, further dilutions should be made in buffer or medium containing carrier proteins, such as albumin or serum.
Storage Condition
Before reconstitution, we recommend a brief spin to drive down any material dislodged from the bottom of the tube. The lyophilized protein should be reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of this protein, further dilutions should be made in buffer or medium containing carrier proteins, such as albumin or serum.
Appearance
Lyophilized in sterile filtered solution of PBS with 2% CHAPS
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Synonyms
Wingless-type MMTV integration site family member 5a, mouse
Description
WNT-5a belongs to the class of WNT proteins that activate the “noncanonical” pathway. The predicted size of mouse WNT-5a is a monomeric protein containing 356 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of ~45 kDa by SDS-PAGE analysis under non-reducing conditions. StemRD’s product is expressed from a mouse cell line, and purified with a proprietary process that is distinct from the published method.
Biological Activity
The activity was determined by using a TCF reporter gene assay in 293 cells co-transfected with Frizzled-4 and LRP-5. WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay (Milkels AJ, et al., PLoS Biol, 4:e115, 2006). This activation mode is utilized because activation assays are generally more reliable than inhibition assays, as they are less prone to any non-specific inhibitory contamination in the preparation.
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