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033-20
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IGF Binding Protein-3 (IGFBP-3), Recombinant (Human)
Catalog#
Standard size
Price
Order
033-20
5 µg
$105.00
MGF (mechano-growth factor)
IGF-I (Insulin-Like Growth Factor I)
Catalog #
033-20
Standard Size
5 µg
Species
Human
Content
This vial contains 5 µg of peptide.
HPLC Analysis
Purity >98% by SDS-Page gel and HPLC analyses.
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Description:
IGF-BP3 is a 30 kDa Cysteine-rich secreted protein. It is the major IGF binding protein present in the plasma of human and animals and it is also found in α-granules of platelets. In addition to its ability to modulate the activity of IGF-I and IGF-2, IGF-BP3 exerts inhibitory effects on follicle stimulating hormone (FSH) activity. Decreased plasma levels of IGF-BP3 often results in dwarfism, whereas elevated levels of IGF-BP3 may lead to acromegaly. The expression of IGF-BP3 in fibroblasts is stimulated by mitogenic growth factors such as Bombesin, Vasopressin, PDGF and EGF. Recombinant human IGF-BP3 is a 28.8 kDa protein consisting of 264 amino acid residues.
Source:
E.Coli
Endotoxin Level:
<1EU/μg
Reconstitution:
Centrifuge the vial prior to opening. Reconstitute in water to a
concentration of 0.1 - 1.0 mg/ml. This solution can be diluted into other
aqueous buffers and stored at 4˚C for 1 week or at -20˚C for future use.
Stability:
The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20˚C. Reconstituted IGF-BP3 should be stored in working aliquots at -20˚C.
Biological Activity:
The ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is ≤0.2 µg/ml in the presence of 15 ng/ml of human IGF-II.
Precautions:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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