Endogenous Ligands for Orphan G Protein-coupled
Receptors, NPFF1 and NPFF2 (HLWAR77) Anti-Neuropeptide AF (Human) (H-048-40)
works for immunohistochemistry in the rat brain tissue!!
Neuropeptide AF (Human) RIA Kit for your research needs!!! Anti-Neuropeptide
AF (Human) IgG is available for western blot analysis!
The anti-opiate Neuropeptides FF and AF (NPFF and NPAF) have been identified
as endogenous ligands for an recently cloned orphan G protein-coupled receptor,
HLWAR77. As HLWAR77 is expressed in brain, adipose and placenta tissues,
C-treminal RF-amide neuropeptides may play other potential pathophysiolgical
roles associated with HLWAR77 receptor.
Elshourbagy N.A., et al. Receptor for the
Pain Modulatory Neuropeptides NPFF and NPAF is an Orphan G-Protein-Coupled
Receptor. JBC (June 12, 2000)
Perry S. J., et al. FEBS Letters 409, 426-430
(1997) g
Identification of neuropeptide FF-related peptides in human cerebrospinal
fluid by mass spectrometry
Several neuropeptide FF (NPFF)-related peptides, known as modulators of
the opioid system, have been previously characterized in bovine and rodent
brain. Reverse-phase high pressure liquid chromatography (HPLC) fractions of
a human with normal pressure hydrocephalus cerebrospinal fluid (CSF),
co-migrating with NPFF-related synthetic peptides, were characterized by
capillary HPLC coupled on-line to nanospray ion trap tandem mass
spectrometry. Two peptides present in the pro-NPFF(A) precursor, NPAF
(AGEGLNSQFWSLAAPQRF-NH(2)) and NPSF (SLAAPQRF-NH(2)), were identified. The
monitoring of NPFF-related peptides in human CSF can be helpful to
understand their roles in pain sensitivity. Burlet-Schiltz O, et al. FEBS
Lett 2002 Dec 18;532(3):313-8
Neuropeptide AF/FF, A New Obesity Related Peptide
Neuropeptide AF/FF has ben shown to modulate a variety of physiological
processes such as food intake, insulin release, blood pressure regulation,
nociception, opiate-induced analgesia, morphine abstinence syndrome and
electrolyte balance. Two G protein-coupled receptors, NPFF1 and NPFF2,
have been identified and characterized as two Neuropeptide AF/FF receptor
subtypes, that are capable of binding NPAF/FF and related peptide in the
nanomolar and sub-nanomolar range.
Among the receptors with the highest
amino acid sequence homology to NPFF1 and NPFF2 are members of the
Orexin, NPY and CCK family, which have been implicated in feeding. These
similarities together with the finding that BIBP3226, an anorexigenic Y1
receptor ligand, also binds to NPFF1 suggest a potential role for NPFF1 in
feeding.
Bonini J.A., et al. Indentification and Characterization of two G protein-
coupled receptors for Neuropeptide FF. JBC, Oct.6, 2000
Pharmacology of Cloned Human and Rat NPFF Receptors
[D-Tyr1-(Nme)-Phe3] NPFF